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T/G HA-VSMC 人血管平滑肌細(xì)胞

簡要描述：CRL-1999 T/G HA-VSMC 人血管平滑肌細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和*培養(yǎng)條件！

產(chǎn)品型號：CRL-1999
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更新時間：2025-09-11
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CRL-1999 T/G HA-VSMC 人血管平滑肌細(xì)胞

ATCC^® Number:

CRL-1999™

Price:

$264.00

Designations:	T/G HA-VSMC
Depositors:	RR Gifford
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens (human)
Morphology:	fibroblast CRL-1999 T/G HA-VSMC 人血管平滑肌細(xì)胞
Source:	Organ: aorta Tissue: smooth muscle Disease: normal
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:	transfection host (technology from amaxa)
Reverse Transcript:	N
DNA Profile (STR):	Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 12 D5S818: 12,13 D7S820: 9,10 THO1: 7,8 TPOX: 8 vWA: 16
Cytogenetic Analysis:	This is a normal human cell line with a 46,XX karyotype. The modal chromosome number was 46, occurring in 90% of cells. The rate of polyploid cells was 0.8%. No structurally altered chromosomes were detected in 15 metaphases karyotyped. Both X chromosomes appeared normal.
Age:	11 months
Gender:	female
Ethnicity:	Caucasian
HeLa Markers:	N
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.05 mg/ml ascorbic acid; 0.01 mg/ml insulin; 0.01 mg/ml transferrin; 10 ng/ml sodium selenite; 0.03 mg/ml Endothelial Cell Growth Supplement (ECGS); fetal bovine serum to a final concentration of 10%, HEPES to a final concentration of 10 mM, TES to a final concentration of 10 mM. Temperature: 37.0°C
Subculturing:	Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, and incubate at 37C for 5 minutes. Add an equal volume of fresh medium, disperse the cells and centrifuge to pellet the cells. Resuspend the cells in fresh medium, aspirate and dispense into new flasks. Subction Ratio: A subction ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days
Preservation:	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004 recommended serum:ATCC 30-2020

CRL-1999

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T/G HA-VSMC 人血管平滑肌細(xì)胞

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