• <bdo id="d74tz"><strong id="d74tz"></strong></bdo>

    <style id="d74tz"></style>

        <ruby id="d74tz"></ruby>

      • 国产无套乱子伦精彩是白视频,亚洲欧美激情精品一区二区,好大好硬好深好爽想要20p,亚洲综合一区二区三区在线,国产一区二区三区啪,久久国产综合精品欧美,欧美成人怡红院一区二区,午夜日本永久乱码免费播放片
        咨詢熱線

        15000266580

        當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-11005FL62891細(xì)胞, 人肝細(xì)胞

        FL62891細(xì)胞, 人肝細(xì)胞

        簡(jiǎn)要描述:FL62891細(xì)胞, 人肝細(xì)胞
        原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

        • 產(chǎn)品型號(hào):CRL-11005
        • 廠商性質(zhì):生產(chǎn)廠家
        • 更新時(shí)間:2025-09-11
        • 訪  問(wèn)  量:2259

        產(chǎn)品分類

        Product Category

        詳細(xì)介紹

        FL62891細(xì)胞, 人肝細(xì)胞

        ATCC® Number:CRL-11005™    Price:$353.00
        Designations:FL 62891

        Depositors:ImClone Systems Inc.

        Biosafety Level:2 [Cells contain SV-40 viral DNA sequences ]

        Shipped:frozen

        Medium & Serum:See Propagation

        Growth Properties:adherent

        Organism:Homo sapiens (human)

        Morphology:fibroblast

        Source:Organ: liver
        Cell Type: immortalized with SV40 large T antigen


        Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
        FL62891細(xì)胞, 人肝細(xì)胞

        Age:fetus

        Comments:The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.

        Propagation:ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 15% fetal bovine serum
        Atmosphere: air, 95%; carbon dioxide (CO2), 5%
        Temperature: 32.0°C


        Subculturing:Protocol:
        1. Remove and discard culture medium.

        2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

        3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

          Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 32°C to facilitate dispersal.

        4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

        5. Add appropriate aliquots of the cell suspension to new culture vessels.

        6. Incubate cultures at 32°C.


        Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
        Medium Renewal: Every 2 to 3 days


        Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
        Storage temperature: liquid nitrogen vapor phase


        Doubling Time:48 hours

        Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

        recommended serum:ATCC 30-2020



        References:70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993
        70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994
        70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996
        70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997
        70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998
        70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999






















        產(chǎn)品咨詢

        留言框

        • 產(chǎn)品:

        • 您的單位:

        • 您的姓名:

        • 聯(lián)系電話:

        • 常用郵箱:

        • 省份:

        • 詳細(xì)地址:

        • 補(bǔ)充說(shuō)明:

        • 驗(yàn)證碼:

          請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
        聯(lián)系方式

        郵箱:xiangfbio@163.com

        地址:上海市虹口區(qū)四平路710號(hào)7層

        咨詢熱線

        400-821-8510

        (周一至周日9:00- 19:00)

        在線咨詢
        • 掃一掃 微信咨詢

        Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
        技術(shù)支持:化工儀器網(wǎng)    管理登陸
        主站蜘蛛池模板: 一区二区三区四区黄色网| 亚洲成a人片在线观看久| 久久天天躁夜夜躁狠狠| 成人区人妻精品一区二区不卡视频 | 中文字幕亚洲无线码一区女同| 国语对白爽死我了| 日本黄页网站免费观看| 婷婷丁香五月深爱憿情网| 国产综合视频一区二区三区| 漂亮的人妻不敢呻吟被中出| 高清在线一区二区三区视频| 亚洲国产精品一二三区| 日本三级香港三级三级人妇久 | 福利一区二区在线观看| 久久久精品免费国产四虎| 免费A级毛片樱桃视频| 无遮挡1000部拍拍拍免费| 日韩人妻少妇一区二区三区 | 亚洲一区二区中文av| 亚洲综合天堂一区二区三区| 亚洲午夜精品国产电影在线观看| 亚洲色大成网站WWW国产| 深夜精品免费在线观看| 免费AV片在线观看网址| 国产 中文 制服丝袜 另类| 亚洲av中文乱码一区二| 91日本在线观看亚洲精品| 白嫩人妻精品一二三四区| 97久久精品人人做人人爽| 丝袜欧美视频首页在线| 久久亚洲精品日本波多野结衣| 99RE6在线视频精品免费下载| 综合亚洲色图| 人成午夜免费大片| 亚洲女同精品一区二区久久| 麻豆国产成人AV在线播放 | 一区二区三区日本久久九| 免费A级毛片无码A∨蜜芽试看| 亚洲综合国产成人丁香五| 伊人久久大香线蕉网av| 亚洲一本大道在线|