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        RAW 264.7小鼠單核巨噬細(xì)胞白血病細(xì)胞

        簡(jiǎn)要描述:RAW 264.7細(xì)胞,小鼠單核巨噬細(xì)胞白血病細(xì)胞 RAW 264.7小鼠單核巨噬細(xì)胞白血病細(xì)胞
        原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
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        • 產(chǎn)品型號(hào):TIB-71
        • 廠商性質(zhì):生產(chǎn)廠家
        • 更新時(shí)間:2025-09-11
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        RAW 264.7細(xì)胞,小鼠單核巨噬細(xì)胞白血病細(xì)胞

        ATCC® Number:  TIB-71™

        Designations:  RAW 264.7

        Depositors:   WC Raschke

        Biosafety Level: 2

        Shipped:  frozen

        Medium & Serum:  See Propagation

        Growth Properties: adherent

        Organism: Mus musculus (mouse)

        Morphology: monocyte/macrophage

        RAW 264.7細(xì)胞,小鼠單核巨噬細(xì)胞白血病細(xì)胞

        Source: Tissue: ascites

        Strain: BALB/c

        Disease: Abelson murine leukemia virus-induced tumor

        Cell Type: macrophage; Abelson murine leukemia virus transformed

        Cellular Products: lysozyme [1207]

        Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


        Applications: Biological response [92560]

        transfection host (Roche FuGENE® Transfection Reagents)

        Receptors: complement (C3) [1207]

        Antigen Expression: H-2d

        Age:  *****

        Gender:  male

        Comments: This line was established from a tumor induced by Abelson murine leukemia virus. They are negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2)This line does not secrete detectable virus particles and is negative in the XC plaque formation assay. The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets.Data communicated in Feb. 2007 by Dr Janet W. Hartley, indicates the expression of infectious ecotropic MuLV closely related, if not identical, to the Moloney MuLV helper virus used in the original virus inoculum. The cells also express polytropic MuLV, unsurprisingly based on the mouse passage history of the virus stocks [ PubMed 18177500].

        Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

        Atmosphere: air, 95%; carbon dioxide (CO2), 5%

        Temperature: 37.0°C

        Subculturing:  Protocol: Subcultures are prepared by scraping.

        For a 75 cm2 flask, remove all but 10 ml culture medium (adjust amount accordingly for other culture vessels). Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels.

        Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

        Medium Renewal: Replace or add medium every 2 to 3 days.

        Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

        Storage temperature: liquid nitrogen vapor phase

        Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

        recommended serum:ATCC 30-2020

        References: 1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

        1207: Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198

        32443: Denlinger LC, et al. Regulation of inducible nitric oxide synthase expression by macrophage purinoreceptors and calcium. J. Biol. Chem. 271: 337-342, 1996. PubMed: 8550583

        32466: Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116

        32553: Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776

        32901: Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306

        33046: Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609

        33076: Lokuta MA, et al. Mechanisms of murine RANTES chemokine gene induction by newcatle disease virus. J. Biol. Chem. 271: 13731-13738, 1996. PubMed: 8662857

        33162: Taylor MF, et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA. J. Biol. Chem. 271: 17445-17452, 1996. PubMed: 8663413

        92560: Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.

        16173094: Hartley JW, et al. Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line. Retrovirology. 4: 5:1, 2008. PubMed 18177500.

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