BB7.2小鼠雜交瘤 B淋巴細(xì)胞

產(chǎn)品型號(hào)：HB-82
廠商性質(zhì)：生產(chǎn)廠家
更新時(shí)間：2025-09-11
訪問(wèn) 量：2746

產(chǎn)品咨詢(xún)聯(lián)系我們

產(chǎn)品分類(lèi)

Product Category

細(xì)胞庫(kù)

細(xì)胞原代牛、狗、雞、鴨、魚(yú)原代細(xì)胞羊原代細(xì)胞豬原代細(xì)胞兔原代細(xì)胞小鼠原代細(xì)胞大鼠原代細(xì)胞人原代細(xì)胞培養(yǎng)基原代細(xì)胞凍存液 ATCC 人乳腺癌豬正常細(xì)胞豬睪丸細(xì)胞系人膠質(zhì)瘤細(xì)胞人成骨細(xì)胞人永生化肝細(xì)胞人肝癌細(xì)胞人卵巢癌細(xì)胞人結(jié)直腸腺癌人經(jīng)典小細(xì)胞肺癌細(xì)胞人小細(xì)胞肺癌細(xì)胞人結(jié)直腸腺癌細(xì)胞人鼻腔上皮細(xì)胞中國(guó)倉(cāng)鼠卵巢懸浮細(xì)胞嗜性逆轉(zhuǎn)錄病毒包裝穩(wěn)定細(xì)胞株小鼠神經(jīng)細(xì)胞瘤人眼脈洛膜黑色素瘤 PT67 逆轉(zhuǎn)錄酶病毒包裝細(xì)胞鴨子胚胎成纖維細(xì)胞豬腎細(xì)胞系昆蟲(chóng)細(xì)胞貓腎細(xì)胞恒河猴胚腎細(xì)胞倉(cāng)鼠卵巢細(xì)胞負(fù)鼠腎細(xì)胞倉(cāng)鼠肺細(xì)胞果蠅胚胎細(xì)胞家貓肺成纖維樣細(xì)胞雞胚細(xì)胞豚鼠心肌來(lái)源細(xì)胞豚鼠肺成纖維樣細(xì)胞豚鼠皮膚成纖維樣細(xì)胞狗腎細(xì)胞非洲綠猴胚胎腎上皮細(xì)胞猴胚胎腎上皮細(xì)胞長(zhǎng)臂猿淋巴瘤新生牛眼囊成纖維細(xì)胞黑化大家鼠肺成纖維樣細(xì)胞俄勒岡地鼠細(xì)胞家兔皮膚成纖維樣細(xì)胞昆蟲(chóng)卵巢細(xì)胞人胸膜瘤細(xì)胞非洲綠猴腎細(xì)胞 VERO 衍生株（SVP）白化金黃地鼠肺成纖維樣細(xì)胞黃胸鼠肺成纖維樣細(xì)胞白鰱尾鰭細(xì)胞蝙蝠肺細(xì)胞草魚(yú)腎細(xì)胞系大黃魚(yú)腎細(xì)胞猴腎細(xì)胞系犬腎細(xì)胞系人結(jié)腸直腸腺癌豬肺泡巨噬細(xì)胞 PFHSA05（TLL2）人重組特洛德樣蛋白因子 PFHSA04 （BMP1）人重組骨誘導(dǎo)因子人乳腺癌細(xì)胞結(jié)腸癌細(xì)胞成纖維細(xì)胞人胚腎細(xì)胞動(dòng)物卵巢細(xì)胞動(dòng)物胚胎細(xì)胞動(dòng)物肺細(xì)胞肋骨來(lái)源細(xì)胞成纖維樣細(xì)胞動(dòng)物上皮細(xì)胞動(dòng)物肝臟細(xì)胞可誘導(dǎo)表達(dá)穩(wěn)定細(xì)胞株動(dòng)物氣管細(xì)胞動(dòng)物腎細(xì)胞動(dòng)物巨噬細(xì)胞人正常細(xì)胞人腫瘤細(xì)胞、癌細(xì)胞小鼠正常細(xì)胞小鼠腫瘤細(xì)胞、癌細(xì)胞大鼠正常細(xì)胞大鼠腫瘤細(xì)胞其它動(dòng)物細(xì)胞查看全部產(chǎn)品

相關(guān)文章

詳細(xì)介紹

HB-82 BB7.2小鼠雜交瘤 B淋巴細(xì)胞 的詳細(xì)介紹

HB-82 BB7.2小鼠雜交瘤 B淋巴細(xì)胞

ATCC^® Number:

HB-82™

Price:

$399.00

Designations:	BB7.2
Depositors:	P Parham
Isotype:	IgG2b
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	suspension
Organism:	Mus musculus （B cell）; Mus musculus （myeloma）（mouse （B cell）; mouse （myeloma））
Morphology:	lymphoblast
Source:	Cell Type: hybridoma: B lymphocyte;
Cellular Products:	immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxic
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HB-82
Comments:	Animals were immunized with papain solubilized HLA A2 antigen. Spleen cells were fused with NS-1 myeloma cells. Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in partial loss of BB7.2 binding. [39935] BB7.2 and PA2.1 （ATCC HB-117） recognize overlapping epitopes on the alpha 2 domain of HLA-A2. [39936] The antibody cross-reacts with cells from various primate species. Tested and found negative for ectromelia virus （mousepox）.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References:	950: Parham P, Brodsky FM. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277-299, 1981. PubMed: 7035415 32263: Cole DJ, et al. T-cell receptor repertoire in matched MART-1 peptide-stimulated peripheral blood lymphocytes and tumor-infiltrating lymphocytes. Cancer Res. 57: 5320-5327, 1997. PubMed: 9393756 39935: Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229 39936: Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666

ATCC® Number: HB-82™ Price: $399.00

Designations: BB7.2

Depositors: P Parham

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus （B cell）; Mus musculus （myeloma）（mouse （B cell）; mouse （myeloma））

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxic

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with papain solubilized HLA A2 antigen.

Spleen cells were fused with NS-1 myeloma cells.

Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in partial loss of BB7.2 binding. [39935]

BB7.2 and PA2.1 （ATCC HB-117） recognize overlapping epitopes on the alpha 2 domain of HLA-A2. [39936]

The antibody cross-reacts with cells from various primate species.

Tested and found negative for ectromelia virus （mousepox）.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 950: Parham P, Brodsky FM. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277-299, 1981. PubMed: 7035415

32263: Cole DJ, et al. T-cell receptor repertoire in matched MART-1 peptide-stimulated peripheral blood lymphocytes and tumor-infiltrating lymphocytes. Cancer Res. 57: 5320-5327, 1997. PubMed: 9393756

39935: Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229

39936: Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666

產(chǎn)品咨詢(xún)

留言框

產(chǎn)品：
您的單位：
您的姓名：
聯(lián)系電話：
常用郵箱：
省份：
詳細(xì)地址：
補(bǔ)充說(shuō)明：
驗(yàn)證碼：

請(qǐng)輸入計(jì)算結(jié)果（填寫(xiě)阿拉伯?dāng)?shù)字），如：三加四=7

上一篇：HB-10745SH-4 鼠雜交瘤細(xì)胞
下一篇：CRL-2016SP2/MIL-6 小鼠骨髓瘤B淋巴細(xì)胞

BB7.2小鼠 雜交瘤 B淋巴細(xì)胞

留言框

產(chǎn)品：

您的單位：

您的姓名：

聯(lián)系電話：

常用郵箱：

省份：

詳細(xì)地址：

補(bǔ)充說(shuō)明：

驗(yàn)證碼：

BB7.2小鼠雜交瘤 B淋巴細(xì)胞